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Sign In Sign In or Sign Up Sign Up Welcome, Guest Applications & Products Applications Cloning & Synthetic Biology DNA Amplification, PCR and qPCR Genome Editing RNA Analysis NGS Sample Prep & Target Enrichment Epigenetics Protein Expression Protein Purification Protein Analysis & Tools Glycobiology & Proteomics Cellular Analysis Product Categories Restriction Endonucleases PCR, qPCR & Amplification Technologies DNA Modifying Enzymes & Cloning Technologies NGS Sample Prep & Target Enrichment Nucleic Acid Purification Markers & Ladders RNA Reagents DNA Assembly, Cloning and Mutagenesis Kits Genome Editing Cellular Analysis Epigenetics Protein Expression & Purification Technologies Competent Cells Protein Tools Glycobiology DNA Plasmids & Substrates Buffers Strains Discontinued New Products Special Offers Product Price List Download a PDF containing pricing for our full product list. You’ll be Thrilled to Pieces Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! Tools & Resources Tools Exo Selector NEBNext ® Selector NEBcloner ® NEBioCalculator ® Thermostable Ligase Reaction Temperature Calculator NEBuilder ® Assembly Tool Enzyme Finder Double Digest Finder NEBcutter ® REBASE ® DNA Sequences and Maps Tool Tm Calculator PCR Fidelity Estimator PCR Selector PolBase ® NEB Golden Gate Assembly Tool NEBaseChanger ® NEBnow Locator Read Coverage Calculator Competitor Cross Reference Resources FAQs Protocols Selection Charts Troubleshooting Guides Usage Guidelines/Tips Interactive Tools Video Library Web Tools Webinars Application Notes NEB Podcast Order Catalog Free Shipping Save time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery. Support Technical Support US Customer & Order Support International Ordering & Support Customer Suggestions & Feedback Find My Local US Sales Representative New Lab/BioTech Discount Catalog & Request Literature NEBnow® Freezer Program NEBsolutions® / OEM Biotech Support Student Services & Materials Packaging & Shipping Quality Assurance Customer Suggestions & Feedback Technical Support Contact Information 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected] About NEB Overview News & Press Releases Leadership Research at NEB Environmental Commitment Quality at NEB ISO Certification Passion in Science Award Business Development Opportunities Careers Contact Us Contact Information 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected] Quick Order Quick Order | | Applications & Products Applications Cloning & Synthetic Biology DNA Amplification, PCR and qPCR Genome Editing RNA Analysis NGS Sample Prep & Target Enrichment Epigenetics Protein Expression Protein Purification Protein Analysis & Tools Glycobiology & Proteomics Cellular Analysis Product Categories Restriction Endonucleases PCR, qPCR & Amplification Technologies DNA Modifying Enzymes & Cloning Technologies NGS Sample Prep & Target Enrichment Nucleic Acid Purification Markers & Ladders RNA Reagents DNA Assembly, Cloning and Mutagenesis Kits Genome Editing Cellular Analysis Epigenetics Protein Expression & Purification Technologies Competent Cells Protein Tools Glycobiology DNA Plasmids & Substrates Buffers Strains Discontinued New Products Special Offers Product Price List Download a PDF containing pricing for our full product list. You’ll be Thrilled to Pieces Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! Tools & Resources Tools Exo Selector NEBNext ® Selector NEBcloner ® NEBioCalculator ® Thermostable Ligase Reaction Temperature Calculator NEBuilder ® Assembly Tool Enzyme Finder Double Digest Finder NEBcutter ® REBASE ® DNA Sequences and Maps Tool Tm Calculator PCR Fidelity Estimator PCR Selector PolBase ® NEB Golden Gate Assembly Tool NEBaseChanger ® NEBnow Locator Read Coverage Calculator Competitor Cross Reference Resources FAQs Protocols Selection Charts Troubleshooting Guides Usage Guidelines/Tips Interactive Tools Video Library Web Tools Webinars Application Notes NEB Podcast Order Catalog Free Shipping Save time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery. Support Technical Support US Customer & Order Support International Ordering & Support Customer Suggestions & Feedback Find My Local US Sales Representative New Lab/BioTech Discount Catalog & Request Literature NEBnow® Freezer Program NEBsolutions® / OEM Biotech Support Student Services & Materials Packaging & Shipping Quality Assurance Customer Suggestions & Feedback Technical Support Contact Information 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected] About NEB Overview News & Press Releases Leadership Research at NEB Environmental Commitment Quality at NEB ISO Certification Passion in Science Award Business Development Opportunities Careers Contact Us Contact Information 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected] Quick Order Sign In Sign Up Practicing Responsible Science From placing the advancement of science and the stewardship of the environment as our highest priorities, to our unique corporate culture, NEB's philosophy can be distilled down to three core values: passion, humility, and being genuine. Read More Find the Support You Need Literature Browse our selection of literature and request your copy of the NEB Catalog and Technical Reference. View or Request Literature Tools Explore our selection of helpful online tools for your research and experimental design. Browse All Tools Video Library Visit our video library to find tutorials, protocols and other informative content. View All Videos Protocol Search Search from over 1,000 NEB protocols within our database. View All Protocols Find the Product You Need Ready to Order? Try our Quick Order feature for faster ordering of products. Quick Order Browse All Products It’s a Matter of Expression. Our NEBExpress™portfolio includes novel solutions for protein expression and purification. NEBExpress™ Ni Spin Columns Ready-to-use pre-packed NEBExpress™ Ni spin columns for rapid purification of His-tagged proteins. Learn More Exo Selector tool Choosing the appropriate exonuclease is simplified when you use our Exo Selector tool. Learn More NEBExpress™ Cell-free E.coli Protein Synthesis System Highly active cell extract system for robust expression of a wide variety of proteins (from 17 to 230 kDa). Learn More Browse All Products Review NEB’s Latest Research Technical Note Learn about NEBNext Enzymatic Methyl-seq (EM-seq), a high-performance, enzyme-based alternative to bisulfite sequencing for methylome analysis. Application Note Access a detailed guide for analyzing nucleic acid concentration and purity with microvolume spectrophotometers. Feature Article Read about the advances in protein expression and purification methodology that have occurred over the past 40 years. NEB Publication Learn more about a highly sensitive loop-medi...